Natural Sciences

Scientific Instrumentation at Hawaii Pacific University

Horgen_lab

Hawaii Pacific University operates a wide range of scientific equipment in support of instruction and research.  The various instruments are housed and maintained by the Shared Instrumentation Facility (SIF), the Instructional Science Laboratories, or by individual investigators. An important strategy in the development of instrumental capabilities at HPU has been to integrate research-grade equipment into our academic curriculum, thereby exposing all undergraduate science majors to state-of-the-art high tech instruments in their laboratory course work.  Collectively, the instrumentation facilities at HPU have been supported by the National Science Foundation, the National Institutes of Health, and private funding. The table below catalogs HPU’s shared instrumentation, their location, and the person to contact for details on availability and potential training.

For more info on the Shared Instrumentation Facility click here.

HPU’s Research Instrumentation

MAJOR INSTRUMENTS

Instrument Description Contact Location
FPLC (fast protein liquid chromatography)
GE Healthcare AKTAprime Plus
Protein purification Boldridge, Melissa MP 401
GC-MS (gas chromatograph-mass spectrometer)
Agilent 7890A GC; 5975C MS
Autosampler; electron impact source; quadrapole analyzer; flame ionization detector Fang, Jiasong HL 214
Infrared spectrometer
PerkinElmer Spectrum Two FT-IR Spectrometer
Measures infrared absorption Allen, Patricia HL 102B
Isocratic HPLC (high-performance liquid chromatography)
Shimadzu LC-6A and D-Star LC; analytical and semipreparative capable; chart integrator, single wavelength UV detector
Purifies extracts or synthetic products Hogg, Grant HL 214
LC-MS (liquid chromatography-mass spectrometer)
Agilent 1200 LC and autosampler; Agilent 6530 quadrapole-time-of-flight (QTOF)
Electrospray and atmospheric pressure chemical ionization, positive and negative mode; MS/MS; high resolution MS. autosampler; fraction collector Hogg, Grant HL 214
NMR spectrometer
300 MHz Varian
1H, 13C, 15N, 31P detection with gradients, one- and two-dimensional experiments Hogg, Grant HL 102D
Patch clamp electrophysiology system
EPC-10 USB Patch Clamp Amplifier; 3 Axis Water Hydraulic Micromanipulator; Olympus IX51; Olympus TC4
Measures flux of ions across cell membranes Chen, Yongli HL 102A
Plate reader
BioTek Synergy HTX Multi-mode Microplate Reader
Fluorescence/absorbance/luminescence plate reader Boldridge, Melissa MP 402
Polarimeter
Rudolph Research Analytical Autopol III
Optical rotation measurements Hogg, Grant HL 102A
Preparative HPLC (high-performance liquid chromatography)
Agilent 1100 preparative system
Flow up to 100 mL/min; manual injector, multi-wavelength UV detector, fraction collector Hogg, Grant HL 214
Scanning robotic plate reader
Molecular Devices FlexStation III
Fluorescence/absorbance/luminescence plate reader with 8 channel fluidics Hogg, Grant HL 102A
Solvent extractor
Thermo Scientific Dionex ASE 350
Extraction of compountd from solid samples Hogg, Grant HL 214
Solvent purification System
Innovative Technology Puresolv
Production of anhydrous solvent Allen, Patricia HL 102B
Spectrofluorometer
JASCO FP-8200
Fluorescence/absorbance/luminescence plate reader Boldridge, Melissa MP 402

CELL AND MOLECULAR BIOLOGY SUPPORT

Instrument Description Contact Location
Autoclave
Yamato SM510
Sterilization of materials and waste Hogg, Grant HL 102A
Autoclave
Yamato SM510
Sterilization of materials and waste Boldridge, Melissa MP 402
Benchtop spectrometer
Bio-Rad Labortories, Smartspec Plus
Measure chemical absorption Allen, Patricia HL 211A
Benchtop spectrometer
Eppendorf BioPhotometer
Measures UV absorption Boldridge, Melissa MP 401
Centrifuge
VWR Clinical 200
Centrifugation of cell culture samples Hogg, Grant HL 102A
Centrifuge
VWR Symphony 2417R
Centrifugation of reagents and samples Boldridge, Melissa MP 401
Centrifuge
Thermo Scientific Sorvall ST40R
Centrifugation of samples Boldridge, Melissa MP 402
CO2 incubator
Sheldon
Controls humidity, CO2 concentrations, and temperature of cell cultures Hogg, Grant HL 102A
CO2 incubator
Thermo Scientific HeraCell Vios 160i
Controls humidity, CO2 concentrations, and temperature of cell cultures Boldridge, Melissa MP 403
Electrophoresis system
Bio-Rad
Separation of DNA and protein Hogg, Grant HL 102A
Electrophoresis system
Labnet Enduro VE10
Separation of DNA and protein Boldridge, Melissa MP 401
Orbital shaker
VWR 3500I
Mixing chemicals and reagents Boldridge, Melissa MP 401
Plate washer
BioTek ELx-50
96 well plate washing, aspirating and dispensing Hogg, Grant HL 102A
Refrigerated centrifuge
Eppendorf Centrifuge 5810 R 15 amp version
Centrifugation of samples (mammalian cells or non-viable samples only) Hogg, Grant HL 214
Shaking incubator
VWR 1575
Heating samples and reagents Hogg, Grant HL 214
Shaking incubator
VWR 1575
Heating samples and reagents Boldridge, Melissa MP 401
Thermocycler
Hybaid, OMN-E Thermal cycler
Incubations and polymerase chain reaction experiments Allen, Patricia HL 102B
Thermocycler
MJ Research PTC-200
Incubations and polymerase chain reaction experiments Hogg, Grant HL 214
Thermocycler
Applied Biosystems Veriti 96 Well Thermal Cycler
Incubations and polymerase chain reaction experiments Boldridge, Melissa MP 402

CHEMISTRY AND BIOCHEMISTRY SUPPORT

Instrument Description Contact Location
Analytical balance
Mettler Toledo PL3001
Measures sample mass Hogg, Grant HL 102A
Analytical balance
Sartorius CP124S
Measures sample mass Hogg, Grant HL 102A
Analytical balance
Sartorius CP124S
Measures sample mass Allen, Patricia HL 102B
Analytical Balance
Sartorius CP124S
Measures sample mass Allen, Patricia HL 212
Analytical balance
Sartorius CP225D
Measures sample mass Hogg, Grant HL 102D
Analytical balance
Sartorius Secura 224-1S
Measures sample mass Boldridge, Melissa MP 402
Benchtop spectrometer
VWR UV-1600PC
Measures UV absorption Hogg, Grant HL 102A
Benchtop spectrometer
VWR UV-1600PC
Measures UV absorption Boldridge, Melissa MP 402
Centrifuge
Beckman Coulter Allegra 6R
Centrifugation of samples Boldridge, Melissa MP 401
Convection oven
Blue-M, MO1450SA
Oven to dry samples Allen, Patricia HL 102B
Convection oven
Lindberg/BlueM, MO1450A-1
Oven to dry samples Allen, Patricia HL 212A
Freeze-dryer
Heto L3300; 6-L capacity
Freeze dry various research sample and dry residual water in samples Hogg, Grant HL 214
Incubator
Benchmark Scientific MyTemp Mini Digital
Oven to dry samples Boldridge, Melissa MP 400
Incubator
VWR BOD Low Temperature Refrigerated Incubator
Incubator for bacterial cultures Boldridge, Melissa MP 401
Oven
Thermolyne F62700 Furnace
Oven to dry samples/equipment Hogg, Grant HL 214
Oven
Fisher Scientific Isotemp
Oven to dry samples Hogg, Grant HL 214
Refrigerated centrifuger
Bechman Coulter
Centrifugation of samples Hogg, Grant HL 102A
Rotary evaporator
Heidolph LR4002
Evaporates solvents from solutions Hogg, Grant HL 214
Rotary evaporator
IKA RV 10 CV S1
Evaporates solvents from solutions Berger, Gideon HL 214
Top loading balance
Sartorius CP2202S
Measures sample mass Hogg, Grant HL 102D
Top loading balance
Denver Instrument SI-403
Measures sample mass Hogg, Grant HL 214
UV/VIS spectrometer
Perkin Elmer Lambda 20
Measure ultraviolet and visible light absorption Allen, Patricia HL 211A
UV/VIS spectrometer
Perkin Elmer Lambda 25
Measure ultraviolet and visible light absorption Allen, Patricia HL 211A
Vacuum evaporation system
Labconco CentriVap Cold Trap
Evaporates solvents from solutions Hogg, Grant HL 214
Vacuum evaporation system
Savant SpeedVac concentrator; -40C cold trap
Evaporates solvents from solutions Hogg, Grant HL 214
Vacuum evaporation system
Welch ChemStar Dry
Evaporates solvents from solutions Hogg, Grant HL 214
Vacuum evaporation system
Welch Self-Cleaning Dry Vacuum System 2028
Evaporates solvents from solutions Hogg, Grant HL 214
Water systems
Millipore Elix and MilliQ
Produces Type II and Type I water Hogg, Grant HL 102A
Water systems
Millipore Elix
Produces Type II water Hogg, Grant HL 102D
Water systems
Millipore Elix and MilliQ
Produces Type II and Type I water Boldridge, Melissa MP 401

ELECTROPHYSIOLOGY SUPPORT

Instrument Description Contact Location
Pipette puller
HEKA RIP 6
Glass pipettes for electrophysiology Hogg, Grant HL 102A
Pipette puller
Narishige PC10
Glass pipettes for electrophysiology Hogg, Grant HL 102A

FREEZERS AND REFRIGERATORS

Instrument Description Contact Location
Freezer
Fisher Scientific Isotemp -86ºC Ultra-Low Temperature
Sample, cell, and reagent storage Hogg, Grant HL 102A
Freezer
Kenmore -20ºC
Sample, cell, and reagent storage Hogg, Grant HL 102A
Freezer
Fisher Scientific Isotemp -20ºC
Sample, cell, and reagent storage Hogg, Grant HL 102D
Freezer
Fisher Scientific Isotemp -20ºC
Sample, cell, and reagent storage Hogg, Grant HL 214
Freezer
Frigidaire -20ºC
Sample, cell, and reagent storage Hogg, Grant HL 214
Freezer
Frigidaire -20ºC
Sample, cell, and reagent storage Hogg, Grant HL 214
Freezer
Fisher Scientific Isotemp -86ºC Ultra-Low Temperature
Sample, cell, and reagent storage Boldridge, Melissa MP 402
Freezer
Kenmore -20ºC
Sample, cell, and reagent storage Boldridge, Melissa MP 403
Refrigerator
Fisher Scientific Isotemp Chromatography
Sample, cell, and reagent storage Hogg, Grant HL 102A
Refrigerator
VWR International, R421FA14
Chemical storage Allen, Patricia HL 102B
Refrigerator
Howard-McCray, HC45
Sample storage Allen, Patricia HL 211A
Refrigerator
Fisher Scientific Isotemp Chromatography
Sample, cell, and reagent storage Boldridge, Melissa MP 401
Refrigerator
VWR Chromatography
Sample, cell, and reagent storage Boldridge, Melissa MP 403
Refrigerator
VWR Chromatography
Sample, cell, and reagent storage Boldridge, Melissa MP 403

FUME HOODS AND BIOLOGICAL SAFETY CABINETS

Instrument Description Contact Location
Biological safety cabinet laminar flow hood
Thermo Scientific 1300 Series A2
Provide aseptic environment for manipulating cells in cultures (for mammalian cells only) Boldridge, Melissa MP 401
Biological safety cabinet laminar flow hood
Thermo Scientific 1300 Series A2
Provide aseptic environment for manipulating cells in cultures (for mammalian cells only) Boldridge, Melissa MP 403
Biological safety cabinet laminar flow hood
Labconco
Provide aseptic environment for manipulating cells in cultures (for mammalian cells only) Hogg, Grant HL 102A
Ductless Fume hood
Cleatech
Clean environment for preparation of PCR and seeding microbial cultures Hogg, Grant HL 214
Fume hood
Iroquois
Safe removal of chemical fumes Boldridge, Melissa MP 401

MARINE BIOLOGY AND OCEANOGRAPHY SUPPORT

Instrument Description Contact Location
CTD
Sea-Bird Electronics, SBE-19-01
Conductivity, temperature and depth for oceanographic water analyses Allen, Patricia HPU, R/V KAHOLO
Current profiler
Nortek USA, Aquadop 1 MHz
Measures marine current Allen, Patricia HL 211A
R/V Kaholo
Murakami Power Catamaran, 42'
Teaching and research vessel Allen, Patricia Kaneohe Bay, Coconut Island
Skiff, motor, trailer
Livingston 12' skiff, 8 hp motor
Research craft Allen, Patricia Kaneohe Bay, Coconut Island

MICROSCOPES AND CAMERAS

Instrument Description Contact Location
Camera
Infinity, 2-3C
General use camera Allen, Patricia HL 213
Camera
Nikon DS-L1
General use camera Allen, Patricia HL 211A
Compound microscope
Nikon E200
General use camera Hogg, Grant HL 102A
Compound microscope
Olympus, CX31
General use camera Allen, Patricia HL 213
Inverted microscope
Nikon TS100
Imaging cells in culture Hogg, Grant HL 102A
Microscope
LW Scientific Revelation III
General use microscope Hogg, Grant HL 102A
Microscope, stereoscope
Nikon SMZ-800
General use dissecting microscope Allen, Patricia HL 211A